• The objective of the experiment was to screen the promising genotypes for tolerance to alkalinity and drought stress as pre-breeding effort for short listing the available gene pool before testing in respective locations.
• To standardize the nutrient medium for in vitro plant formation from nodal / shoot apex explants of promising mulberry genotypes.
• To develop an efficient protocol for regeneration through indirect organogenesis from different explants (leaf, internode segment and petiole) as this is a pre-requisite for various biotechnological approaches in tropical genotypes.
• The objective of the project is to develop mulberry transgenics over expressing transcription factors associated with drought and epicuticular wax biosynthesis to increase stress tolerance and leaf quality.
• To develop doubled haploid lines in promising mulberry genotypes through induction of gynogenesis in vitro.

• In in vitro screening of mulberry genotypes for abiotic stress tolerance conditions (salinity, alkalinity and drought), the protocol has been standardized. So far 157 genotypes have been screened. Twenty two genotypes were identified for tolerance to alkalinity and salinity. Twelve genotypes were identified for tolerance to drought.
• The protocol for the micropropagation of elite mulberry genotypes viz., V-1, S-36, S-34, S-13, AR-12 and AR-11 have been developed through nodal explants / shoot apex cultures.
• Standardized the protocol for obtaining regeneration from leaves culture of 5 elite mulberry genotypes viz., V-1, S-36, S-34, S-13 and AR-12. The regenerated shoots were subsequently hardened and transferred to field.

Regeneration from leaf explant culture	Rooting of in vitro regenerated shoot



• Young leaves collected from V-1, S-34 and S-36 genotypes were used for Agrobacterium-mediated transformation. Agrobacterium-mediated co-cultivation was undertaken using the construct mod. pBin. The vector contains DREB genes (Dehydrated Element Binding gene) and selection was done on the respective best suited regeneration media.
• Female flowers of selected promising genotypes were cultured on different media to induce gynogenesis.

• In vitro screening of genotypes for various abiotic stress (drought, salinity and alkalinity).
• Standardization of protocol for obtaining regeneration from different explants to use further in various biotechnological approaches in tropical genotypes.
• Genetic transformation in mulberry

1. Development of mulberry transgenics over expressing transcription factors associated with drought and epicuticular wax biosynthesis to increase stress tolerance and leaf quality (DBT funded - (In collaboration with UAS, GKVK, Bangalore)

• Training
• Short term dissertation / Ph.D guidance

1. Culture room
2. Inoculation room
3. Laminar air flow, Distillation unit, Oven, Incubator shaker, Centrifuge, Osmometer, Moisture balance, pH meter, B.O.D. incubator, Microscopes.

Twenty (20).

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